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Briefly, 10 to 15 mg of muscle tissue was homogenized with a manual micropestle in 20 mM potassium phosphate buffer pH 7.5, and debris were discarded by maximum speed centrifugation, at 4°C. 100 ml of sample was mixed by vortex with 330 μl of n-propanol in the presence of 0.5 mM β-mercaptoethanol. Sample debris were discarded after spin at maximum speed and clean lipid extracts were

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Operating Procedures for the UGA Geology Bruker D8 Advance X-ray Diffractometer Start-up (X-ray warning lights on top of cabinet are on and water chiller is running) This is the state that the diffractometer should be most often found.

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Page 1 of 17 Bruker D8 HRXRD Basic Operation of the Bruker D8 HRXRD and XRD Commander SOP for configuring the Bruker D8 HRXRD, how to use XRD

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treadmill training, cycle training or an untrained group. Lower hindlimb muscle size was examined prior Lower hindlimb muscle size was examined prior to SCI and at 1-, 2-, 4-, 8-, and 12-week post

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the treadmill speed was increased by 2 m/min every 120 s until exhaustion was reached. To encourage the mice to run, an electric shock grid at the base of the treadmill was activated to deliver a 0.2-mA pulse. This delivered an uncomfortable shock but did not injure or harm the mice. The maximal running speed was considered the maximal workload capacity of the animal (13, 26). Grip strength

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Operating Procedure for Bruker D8 Discover X-ray Diffractometer . Preparation: • Log into login computer • Check System Control Buttons (located at the lower right or left of the system.

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6 D8 ADVANCE– understanding the sample behind the signal X-ray diffraction (XRD) is the ideal, non-destructive analytical method for examining all types of samples!

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Bruker D8 Discover XRD User’s Guide I. Powder Diffraction Measurement using LynxEye Detector by Susheng Tan, Ph.D. NanoScale Fabrication and Characterization Facility, University of Pittsburgh M104 Benedum Hall, 3700 O’Hara Street, Pittsburgh, PA 15261 …

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The D2 PHASER is a portable desktop XRD instrument for research and quality control. It is easy to operate and independent of external media such as cooling circuits. Thanks to the LYNXEYE detector it is the fastest desktop XRD system on the market.

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